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Negative control sections were prepared by omitting of the primary antibodies, preadsorbing the primary antibodies with an excess nmol L of the pure antigen when available, and mismatching secondary antibody. After incubation for,,, and minutes at degrees C, reactions were stopped with mu l of wt vol sodium acetate, pH. We next pretreated GBM SN with neutralizing anti TGF beta sub antibody before exposure to HUVECs and found a reduction in lance increase p.
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